Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal logo zp2

Zebrafish phenotyping platform

Establishment of in vivo infectious models (BSL2)

viral and bacterial pathogens
viral and bacterial pathogens

  Zebrafish is now recognized as an emergent used in applied biomedical researches. Beyond its remarkable optical transparency, this small vertebrate provides an ideal, cheap model to support high content screenings of therapeutic molecules in complementary to rodent models. So far, zebrafish larvae contribute to investigate host-pathogen interactions in real time by using high-resolution intravital imaging. Several bacterial and viral disease models have thus been developed taking advantage of the genome editing tools, transgenic lines and mutants available in the zebrafish ressource centers. These studies provided important knowledges for the identification of biomarkers and the development of effective therapeutics against bacterial and viral pathogens in human health and economically important fishes.

The IERP platform contributed to the establishment of fish and human viral infectious models, in collaboration with the immunologists of the Virology and Molecular Immunology (VIM) unit. These researches have resulted in the development of tools for the study of pathogen invasion and antiviral innate immune responses (at the molecular and cellular level). Collaborative projects are underway to set up bacterial models, which will benefit from the available tools of the structure (transgenic lines and morpholinos).

           The platform benefits from a strong local expertise in (fish) virology, bacteriology and immunology and accessibility to L2 laboratorie. We develop innovative strategies to study infectious diseases combining advanced imaging approaches (2-photon imaging, 3d/3d+t microscopy), screening approaches (multi-channel, multi-position fast imaging, data processing), daily monitoring (slowing blood circulation, tissue damage/regeneration, behavioral disorders, ..) and nanotechnologies (for molecule delivery or telemetry). Collaborations can be established to evaluate candidate molecules in advanced screening assays on relevant zebrafish lines for toxicity (craniofacial deformation, neurotoxicity, reproductive system disorders, inflammation), and/or bioactivity (anti-inflammatory effect, anti-infectious …).

                 Services can be provided to :

  • establish novel infectious models (microinjection- infectious disease characterization: clinical signs, survival curve, behavioral deficits)
  • study pathogen invasion (tracking of fluorescent pathogens, injections of pathogenic mutants, screening of anti-infectious candidates ....)
  • characterize host-pathogen interactions (innate immune responses: immune cells dynamic, cytokines expression, tissue damage and regeneration…)
  • conduct functional experiments: gain or loss of functions through mRNA or morpholino injections

                 A collection of pathogens at the service of our academic and industrial partners: viruses, bacterila, prions...

The collaborative research units (INRAE, ANSES, CIRAD) backed by the Emerg'in infrastructure possess an unique collections of viral, bacterial and parasitic pathogens affecting farm animals and humans ( zoonotic agents) in addition to the microbial ressource center CIRM International Center for Microbial Resources for Pathogenic Bacteria (CIRM-BP).