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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

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Zebrafish phenotyping platform

Apports des techniques d'imagerie en trois dimensions pour l'étude des interactions hôtes-pathogènes

 Apports des techniques d'imagerie en trois dimensions pour l'étude des interactions hôtes-pathogènes
© PerkinElmer
Apports des techniques d'imagerie en trois dimensions pour l'étude in vivo/ ex vivo des interactions hôtes-pathogènes

Christelle Langevin

Chercheur INRAE- Unité d’infectiologie expérimentale rongeurs et poissons

Responsible scientifique du plateau de phénotypage par imagerie in vivo et transparisation. 

INRAE Jouy en Josas. 

 

Notre équipe développe des modèles expérimentaux et des méthodes de phénotypage pour l’étude des interactions hôtes-pathogènes in vivo. Nous modélisons et étudions des pathologies infectieuses (humaines et animales) en poisson zèbre et en souris. Notre expertise en imagerie nous permet d’assurer le suivi in vivo des processus infectieux et des réponses immunitaires par imagerie intravitale sur ces différentes espèces modèles. Plus récemment, nos travaux ont illustré l’apport des techniques de transparisation et d’imagerie 3D pour l’étude des pathologies infectieuses sur organe entier à haute résolution. Les applications sont nombreuses notamment en terme de diagnostic et de criblage de molécules thérapeutiques.

See also

New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution. Frétaud M, Descamps D, Laubreton D, Rameix-Welti MA, Eléouët JF, Larcher T, Galloux M, Langevin C. Viruses. 2021 Jan 28;13(2):201. doi: 10.3390/v13020201. PMID: 33525646 Free PMC article. New reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development. Frétaud M, Do Khoa N, Houel A, Lunazzi A, Boudinot P, Langevin C. Dev Dyn. 2021 May;250(5):701-716. doi: 10.1002/dvdy.286. Epub 2021 Jan 19. PMID: 33369805  Respiratory syncytial virus tropism for olfactory sensory neurons in mice. Bryche B, Frétaud M, Saint-Albin Deliot A, Galloux M, Sedano L, Langevin C, Descamps D, Rameix-Welti MA, Eléouët JF, Le Goffic R, Meunier N. J Neurochem. 2020 Sep;155(2):137-153. doi: 10.1111/jnc.14936. Epub 2020 Jan 8. PMID: 31811775 A DNA Prime Immuno-Potentiates a Modified Live Vaccine against the Porcine Reproductive and Respiratory Syndrome Virus but Does Not Improve Heterologous Protection. Bernelin-Cottet C, Urien C, Fretaud M, Langevin C, Trus I, Jouneau L, Blanc F, Leplat JJ, Barc C, Boulesteix O, Riou M, Dysart M, Mahé S, Studsrub E, Nauwynck H, Bertho N, Bourry O, Schwartz-Cornil I. Viruses. 2019 Jun 25;11(6):576. doi: 10.3390/v11060576. PMID: 31242645 Free PMC article. Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus. Bordet E, Frétaud M, Crisci E, Bouguyon E, Rault S, Pezant J, Pleau A, Renson P, Giuffra E, Larcher T, Bourge M, Bourry O, Boulesteix O, Langevin C, Schwartz-Cornil I, Bertho N. Front Immunol. 2019 May 3;10:953. doi: 10.3389/fimmu.2019.00953. eCollection 2019. PMID: 31130951 Free PMC article.