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Last update: May 2021

WP2 - Spatial descriptors

Spatial descriptors at the soil pore scale

Coordinator: Wilfred Otten _ Main Participants: David Montagne, Marine Lacoste, Philippe Baveye,Naoise Nunan, Xavier Raynaud, Claire Chenu, Sonja Schmidt, Valérie Pot, Olivier Monga, Ruth Falconer, 1 master student - Marine Lacoste will stay for 3 weeks in Simbios, David Montagne and Naoise Nunan will stay for 1 week

The objective of WP2 is to characterize the soil systems used in WP1 in different ways, and expand on this characterizations with simulated data associated with a range of scenarios, to pave the way for the modelling work to be carried out in WP3.

Specifically we shall:

Characterise the physical habitat in soil samples: We shall use X-ray μCT to visualize and quantify the spatial organisation of pores and solids at scales relevant to microorganisms,
Evaluate the pore-scale distribution of water, particulate organic matter, and microorganisms in soil samples, using respectively i) experimental and modelling procedures to characterise the spatial distribution of water within pores, ii) a combination of X-ray CT, staining methods, neutron scanning, and mathematical methods to quantify the spatial organisation of POM in soil, iii) thin sectioning and microscopic techhniques to locate micro-organisms within the soil microenvironment.
Expand the range of situations that can be considered, by simulating different scenarios (in terms of location of microorgansisms or organic matter, and moisture level), based on the physical data obtained in step 1.
Evaluate descriptors of soil microheterogeneity, for use in WP4.

**WP2.1 Characterisation of the physical structure by X-ray μCT: (Main : W. Otten)**

WP2.2 : Characterisation of organic matter distribution: (Main : C. Chenu)

**WP2.3 : Distribution of microorganisms in soils: (Main : P. Baveye, N. Nunan)**

**WP2.4 Water distribution: (Main : V. Pot, R. Falconer)**

**WP2.5: Extension of experimental data set with alternate scenarios(Main : Sonja Schmidt)**

**WP2.6 : Spatial descriptors of soil microscale heterogeneity (Main : D. Montagne, M. Lacoste, 1 master student)**

Deliverables :

- 3D data related to pore geometry and the distribution of solids, water, POM, bacteria, and fungal hyphae in samples used in lab.-based experimentation (WP1), as well as in virtual soil samples corresponding to a range of alternate scenarios. These data will be used to parameterise the models in WP3

- Various initial conditions to be used for model simulations within ranges that can be expected under experimental conditions

- Descriptors of spatial distribution of solid, water, microorganisms, in soils, for use in WP4