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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Institutions

SPS - Saclay Plant Sciences

Adaptation to Nitrogen Availability in Arabidopsis and Brachypodium (AdaptN)

Coordinator: Anne Krapp

PhD project selected in 2012 – Person recruited: Camille Chardin

Nitrogen is one of the most important macronutrients for plants. In the soils, nitrogen exists under different organic and inorganic forms. Plants preferentially use nitrate, which is often in limiting amount in soils. Recently, it was shown that the NLP7 transcription factor is a master regulator of the primary nitrate response and that the NPF6.3 nitrate transporter plays a role in the nitrate signal sensing. By studying the simple and double mutant’s biomass and sentinel gene regulation in response to nitrate, we observed additive phenotypes. In addition we showed that the nuclear relocation mechanism of NLP7 in response to nitrate is still active in the npf6.3 mutant background. All together, these results showed that NLP7 and NPF6.3 are not in the same signalling pathway. Only a few regulators of the nitrate response are known. Interestingly the direct target genes of NLP7 are highly enriched for regulatory proteins such as other transcription factors or protein kinases, which places NLP7 at a high hierarchical place in the nitrate signalling pathway. In order to identify molecular events downstream of NLP7, we studies two NLP7 direct targets. In protoplasts, we have shown that MAPKKKK13/14 are able to activate Group C MAPKs via MKK3. In addition we showed in planta that nitrate addition indeed triggered the activation of group C MAPKs and that this activation is dependent on NLP7 and MKK3. Furthermore we obtained first indications that nitrate-dependent developmental traits are partially impaired in the single mutants mapkkk13 and mapkkk14, and the double mutant mpkkk13mpkkk14.


Chardin C., Girin T., Roudier F., Meyer C., Krapp A. (2014). The plant RWP-RK transcription factors: key regulators of nitrogen responses and of gametophyte development. Journal of Experimental Botany 65(19): 5577-5587.

Chardin C., Schenk S.T., Hirt H., Colcombet J., Krapp A. (2017). Review: Mitogen-Activated Protein Kinases in nutritional signaling in Arabidopsis. Plant Sci 260: 101-108.

Girin T., David L.C., Chardin C., Sibout R., Krapp A., Ferrario-Mery S., Daniel-Vedele F. (2014). Brachypodium: a promising hub between model species and cereals. Journal of Experimental Botany 65(19): 5683-5696.

Krapp A., David L.C., Chardin C., Girin T., Marmagne A., Leprince A.-S., Chaillou S., Ferrario-Mery S., Meyer C., Daniel-Vedele F. (2014). Nitrate transport and signalling in Arabidopsis. Journal of Experimental Botany 65(3): 789-798.

Communications in conferences:

Camille Chardin - PhD student retreats

Anne Krapp - Plenary lecture at Nitrogen 2016, Montpellier