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Menu Institutions

SPS - Saclay Plant Sciences

The Evos microscope

A teaching tool

Thanks to SPS funding, the teaching team associated with the LabEx has recently acquired an EVOS digital microscope dedicated to education and available since September 2015. This equipment is located in the IPS2 building dedicated to teaching (building 630).

A microscope particularly adapted to teaching and training

Microscope Evos

The EVOS microscope has not eyepiece but a display screen, which makes it easy to use. It thus allows demonstrations and observations in small groups in a more convivial context. Compact and robust, it can be moved easily and it is suitable for beginner users. This inverted epifluorescence microscope makes it possible to work in teaching laboratories as the presence of a strong natural light does not interfere with its operation. Equipped with LED technology, the lamps have a very long lifespan which significantly reduces the maintenance costcosts.

A few examples of use

Cytoskeleton immunostaining - Licence 2 (second year of the French 3-year bachelor’s degree)

The microscope was used in 2015 in a series of practical courses for a “Licence 2” teaching module about “Animal and plant cellular biology”. The experiment consisted in marking mouse fibroblasts using antibodies, in order to highlight the actin filaments and the microtubule network in cells in interphase and in division. Additional marking with DAPI also revealed nuclear DNA. Thus, each pair of students could observe the results of their own experiments and analyze them independently. The resulting images were then manipulated through the ImageJ software. The students assembled their observations and presented an illustrated plate in the waystyle of scientific articles.

Chromatin immunostaining - Master 1 (first year of the French 2-year Master’s degree)

Within the frameframework  of a “Plant development" teaching module for the Plant sciences “Master 1”, a new practical course was designed throughbecause of the acquisition of the EVOS microscope. The objective will beis to compare the chromatin organization within different tissues and / or different organisms through the use of antibodies targeting post-translational modifications of histones specific to either heterochromatin or euchromatin. On one hand, theseThese marks will be compared between different seedling tissues of the Arabidopsis model plant. On the other hand, they will be and studied in different crop species such as tomato and wheat. The aim will beis to understand the evolution and control of chromatin reorganization and the potential impacts on the regulation of genome expression. Students will have to synthesizeproduce their results, then present them and analyze them in the form of an oral presentation.

Development of organisms - Licence 1 (first year of the French 3-year bachelor’s degree)

Optional teaching module - Ongoing project

The objective of this practical course will be to observe the effect of auxin on development by tracking a fluorescent marker of auxin (DR5::GFP) in response to hormonal treatment.

Some technical features

  • 3 types of filters are available for use inwith RFP, GFP and  DAPI
  • Available lenses: 10x aperture 1.2 infinity-corrected, 40x 0.30, 1x50 immersion, 100x immersion. The 10x and 40x lenses make it possible to perform phase-contrast acquisitions.
  • Several vessel holders are available: Petri dishes of different diameters, microscope slides, etc.
  • 3 USB ports for backups
  • Possibility of connecting a keyboard
  • Natural light is strongly attenuated by a case (cover)
  • 12-bit coding with 16-bit encapsulation, allowing to work with Bio-imaging software like ImageJ.

Contact

Frédéric Coquelle
I2BC
frederic.coquelle[at]i2bc.paris-saclay.fr