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SPS - Saclay Plant Sciences

New UHPLC-HRMS / MS on the Plant Observatory Chemistry and Metabolomics platform

A mass spectrometer coupled to a liquid chromatography system

Early in 2015, the Plant Observatory Chemistry and Metabolomics platform located on the INRA Center of Versailles-Grignon acquired a new device: the UHPLC-HRMS.

On this occasion, we met Gregory Mouille, Research Director at the Institut Jean-Pierre Bourgin of Versailles and scientific manager of this platform, as well as of the Metabolomic platform of the Institute of Plant Sciences Paris-Saclay.

UHPLC-HRMS/MS

A very high resolution device to analyze molecules

"The UHPLC-HRMS / MS is composed of a mass spectrometer with quadrupole analyzer, coupled with a time of flight analyzer (Bruker QTOF Impact II), and a Ultra-High Performance Liquid Chromatography instrument (Dionex UHPLC Ultimate 3000 Thermo). This machine will be used to analyse molecules of plants. It allows us to evaluate the accurate mass of the molecules and help to determine their structure and to characterize them. The HRMS high resolution instrument offers a very high mass accuracy and gives the possibility to propose molecular formulas for the analyzed molecules. With this device, the technological equipment of the Plant Observatory is now optimal." enthuses Gregory.

Extrait de feuilles d'Arabidopsis par l'UHPLC-QTOF

Figure : Arabidopsis leaves extract analyzed by a UHPLC-QTOF (Bruker IMPACT II), a) total ion current  and ion m/z 434.0619 chromatograms, b) mass spectrum for the 4.5 min peak and identification of the empirical formula, corresponding to 5-methylthiopentyl glucosinolate

The UHPLC-HRMS / MS can be used for analyzes in the area of agriculture and food but currently many requests come from companies in the sector of cosmetics and pharmaceutics.

Managing more efficiently the acquisition of equipment and pooling needs

Once a year, the SPS LabEx Infrastructure group evaluates funding applications for large equipment from SPS researchers. Then SPS contributes to a better coordination of needs and purchases between laboratories. Concerning the UHPLC-HRMS/MS, the purchase was co-financed by the “Région Ile-de-France”, INRA, IBISA and the SPS LabEx.

Belonging to a network such as the SPS LabEx provides access to a wider choice of equipment distributed between several laboratories. The instruments, available for the researchers of the institutes, are also open to the entire scientific community.

How to benefit from the UHPLC-HRMS ?

To benefit from a service / collaboration, contact the scientific manager of the platform:

Grégory Mouille
Plateforme de Chimie/Métabolisme de l’Observatoire du Végétal
Bâtiment 1
Institut Jean-Pierre Bourgin
Centre Inra de Versailles-Grignon
Route de St-Cyr (RD 10)
78026 Versailles Cedex
France

pfcv[at]versailles.inra.fr
+33 (0)1 30 83 33 39

Website

SPS webpage of the platform

Chemistry and Metabolomics platform website

Plant Observatory website