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Last update: May 2021

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PVY Organization

Zone de texte éditable et éditée

Poland

Plant Breeding and Acclimatization Institute - National Research Institute, Młochów Research Center

Poland
The Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (PIB) is the largest Polish research centre in the multi-disciplinary area of crop improvement, biotechnology, germplasm conservation and enhancement. IHAR was founded in 1951 for research in breeding and seed production of major field crops. Młochów Research Center is dedicated on the research on identification of genetic sources and the mechanisms of potato resistance to pathogens and abiotic stresses, enhancement of breeding for important agricultural traits, characterization and collection of Solanum plants and pathogens of potato.

Missions                                                     

The main research of IHAR related to the PVY research  team including: providing the PVY resistant breeding material to potato breeders, monitoring the PVY population changes in potato production, characterization of PVY resistance, studies on Virus –host interaction

Research topics covered:

  • Pre-breeding for PVY resistance
  • PVY population diversity in Poland
  • PVY strain identification and characterization
  • Antagonistic interactions between PVY  isolates in plants
  • Resistance genes to PVY - extreme resistance (ER) and hypersensitive resistance (HR).
  • Evaluation of PVY resistance of new potato cultivars
  • Proteomic comparison of potato genotypes with ER- and HR-mediated resistance to PVY
  • Host microRNA studies in potato-PVY, tobacco-PVY pathosystems

Contact

Dr Zhimin Yin (Head of the team)

Head team

Email: z.yin@ihar.edu.pl

Tel: (+48 22) 729 92 48 ext. 210  

 

                                             

Skills                                            

  • Detection of potato viruses (biological, ELISA, RT-PCR)
  • Real-time RT-qPCR
  • Sequencing
  • Western blotting
  • Two-dimensional gel electrophoresis
  • Liquid chromatography-tandem mass spectrometry
  • microRNA detection by stem-loop real-time RT-qPCR

Team                               

Five people staff (from left to the right):

  • Dr Zhimin Yin;
  • Dr Katarzyna Szajko;
  • M.Sc. Krystyna Michalak
  • Professor Bogdan Flis (M.Sc. Anna Grupa not present)
team

                                                                                               

Selected publications

  • Flis B, Hennig J, Strzelczyk–Żyta D, Gebhardt Ch, Marczewski W (2005). The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding, 15: 95 – 101
  • Yin Z, Chrzanowska M, Michalak K, Zagorska H, Zimnoch-Guzowska (2012) Recombinants of PVY strains predominate among isolates from potato crop in Poland. Journal of Plant Protection Research, 52(2): 214-219.
  • Zimnoch-Guzowska E, Yin Z, Chrzanowska M, Flis B, (2013). Sources and effectiveness of potato PVY resistance in IHAR’s breeding research. American Journal of Potato Research. 90: 21-27
  • Yin, Z. Xie F, Michalak K, Pawełkowicz M, Zhang B, Murawska Z, Lebecka R, Zimnoch-Guzowska E (2016) Potato cultivar Etola exhibits hypersensitive resistance to PVYNTN and partial resistance to PVYZ-NTN and PVYN-Wi strains and strain-specific alterations of certain host miRNAs might correlate with symptom severity. Plant Pathol. DOI:10.1111/ppa.12599
  • Szajko K, Strzelczyk-Żyta D, Marczewski W (2017) Comparison of leaf proteomes of potato  (Solanum tuberosum L.) genotypes with ER- and HR-mediated resistance to PVY infection,  European Journal of Plant Pathology, on-line first