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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

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PVY Organization

Zone de texte éditable et éditée

France

France
The French Federation of Seed Potato Growers (FN3PT) gathers the seed potato growers established across three areas: the North, the West and the Center of France, which depend on three regional structures Comité Nord, Bretagne-Plants, and Comité Centre et Sud, respectively. The seed potato production in France covers about 20,000 ha, and approximately 500,000 certified tons.

The FN3PT, by delegation of the Ministry of Agriculture (GNIS-SOC), is responsible for the inspection and the certification of seed potatoes concerning the varietal identity and purity, health quality and physiological status. In addition, main actions of FN3PT and its regional organization are the research and innovation on seed potatoes, production of starting material, breeding of new varieties and promotion of the sector.

For more than 30 years,FN3PT has developed some collaborative research programs with INRA-IGEPP (French Institute for Agriculture Research) which has been formalized by the creation in 2012 of a Joint Technological Unit called InnoPlant in order to promote innovation and to increase the competitiveness of the French seed potato industry.

Contact                             

Laurent Glais (Head of the team)

LG

Email: laurent.glais@fnpppt.fr 

      or laurent.glais@inra.fr

  Tel.: 33 (0)2 23 48 70 28

Equipe Viro 2016

Three people staff 

  • 1 Research engineer (Laurent Glais, on the left)
  • 2 Assistant engineers:

              * Maryse Guillet (on the middle)

              * Frédéric Boulard (on the right side)

Missions of the team

The main objectives of our team are to meet the needs of the seed potato growers and to give to the French certification laboratories the means, in terms of diagnostic tools and knowledge, to assume the health quality of seed potato. To meet these requests, we carry out different research programs, mainly applied on PVY:

  • production and supplying of serological reagents (polyclonal, monoclonal antibodies) to the laboratories for the inspection and certification of French seed potatoes (PVY, PLRV, PVX)
  • development  and adaptation of new diagnostic tools (serological, molecular)
  • study of PVY diversity in France and elsewhere, on potato and tobacco host plants
  • identification of PVY viral molecular determinants involved in the expression of the necrosis on tobacco leaves and on potato tuber (PTNRD)
  • characterization of resistance genes (N) involved in PVY/potato interactions
  • study of PVY load in potato tubers given the inoculation date in field
  • expertise and analyses on various potato viruses

Skills                                                 

  • Immunology
  • Phenotyping
  • Virus diagnosis
  • Aphid identification
  • ELISA
  • qRT-PCR
  • RPA
  • Reverse genomic

Selected publications

  • Glais, L., Faurez, F., Boulard, F., Tribodet, M., Jacquot, E. (2015). The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers. Virus Research, 208, 110-119. pdf
  • Glais, L. and Jacquot, E. (2015). Detection and characterization of viral species/sub-species using isothermal Recombinase Polymerase Amplification (RPA) assays. In C. Lacomme (Ed.), Methods in Molecular Biology, Plant Pathology Techniques and Protocols: Humana Press.
  • Janzac B., Willemsen A., Cuevas J. M., Glais L., Tribodet M., Verrier J.L., Elena S.F. and Jacquot E. (2015). Brazilian Potato virus Y isolates identified as members of a new clade facilitate the reconstruction of evolutionary traits. Plant Pathology, 64, 799-807. pdf
  • Lacroix C., Glais L., Kerlan C., Verrier J.L. and Jacquot E. (2010). Biological characterization of French Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants possessing the recessive resistance gene va. Plant Pathology 59 (6): 1133-43. pdf
  • Glais L., Tribodet M. and Kerlan C. (2002). Genomic variability in Potato potyvirus Y (PVY): evidence that PVYNW and PVYNTN variants are single to multiple recombinants between PVYO and PVYN isolates. Archives of Virology 147: 363-378. pdf