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Last update: May 2021

Menu Logo Principal

PVY Organization

Zone de texte éditable et éditée

Canada

Agricultural Certification Services Inc./Potatoes NB

Canada
ACS is a not-for-profit, Canadian Food Inspection Agency (CFIA) approved diagnostic lab owned by the potato growers of New Brunswick. Located in Fredericton, NB, ACS has been in operation since 1996 and provides testing services to potato industries across Canada. ACS also provides testing for different strawberry viruses.

Missions of the team

Development of detection procedures using nucleic acids, gene cloning and sequencing including design of oligonucleotide primer, synthesis, purification and quantification, Reverse Transcription-Polymerase Chain Reaction (RT- PCR) and Real-Time PCR, agarose and polyacrylamide gel electrophoreses, enzyme-linked Immunosorbent Assay (ELISA).

Currently focused on development of diagnostic techniques for potato diseases particularly on detection and management of Dickeya, PVY and Verticillium.

Skills

ACS is equipped to do microbiological, serological and molecular research work and uses new technologies for testing, such as PCR and real-time PCR along with ELISA.

Contact

Dr. Mathuresh Singh (Head of the team)

Matturesh Singh

Email: msingh@potatoesnb.com

Tel.: 506-459-0293

Selected publications

  1. MacKenzie TDB, Lavoie J, Nie X and Singh M. 2017. Effectiveness of combined use of mineral oil and insecticide spray in reducing Potato virus Y (PVY) spread under field conditions in New Brunswick, Canada. Am J Potato Res 94: 70-80 pdf
  2. MacKenzie TDB, Nie X and Singh M. 2016. Crop Management practices and reduction of on-farm spread of Potato virus Y: a 5-year study in commercial potato fields in New Brunswick, Canada. Am J Potato Res 93: 552–563 pdf
  3. Fageria M, Nie X, A Gallagher and Singh M. 2015. Mechanical transmission of Potato virus Y (PVY) through seed cutting and plant wounding. Amer. J. Potato Res. 92:143–147
  4. MacKenzie TDB, Nie X and Singh M. 2015. RT-PCR and Real-Time RT-PCR methods for the detection of Potato virus Y in potato leaves and tubers. Plant Virology Protocols - Methods in Molecular Biology 1236: 13-26
  5. MacKenzie TDB, Fageria MS, Nie X and Singh M. 2014. Effects of crop management practices on current season spread of Potato virus Y. Plant Dis. 98: 213-222
  6. Fageria MS, Singh M, Nanayakkara U, Pelletier Y, Nie X and Wattie D. 2013.  Monitoring current season spread of Potato virus Y in potato fields using ELISA and real-time RT-PCR.Plant Dis. 97:641-644.
  7. Singh, M., Singh, R. P., Fageria, M. S., Nie, X., Coffin, R., and Hawkins, G. 2013. Optimization of a Real-time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. Am. J. Potato Res. 90:43-50.