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24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

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Microgenomics International Symposium 2014

View the Presentations

Click on the pdf of the presentations you would like to see and download it. Some authors have requested that only a shortened version of their presentation be available on the web.

Thursday, May 15 2014








Welcome / Introduction (organizer)



Session 1: Sampling methods (flow cytometry, laser capture microdissection, DEP array, Single cell picking ...)

Chariman: Marc Dalod


Keynote lecturer

Characterization of very rare immune cell types through their purification and gene expression profiling : different tools and strategies to obtain pure or enriched cell populations,  advantages and disadvantages of each tool and their complementarily)

Marc Dalod, CNRS-Centre d'immunologie de Marseilles-Luminy France


Invited lecturer

DeCIPHEr critical steps in a flow cytometry experiment to ensure reproducible and high quality microgenomic datatsets

Hervé Luche, Immuno-Phenotyping Module, Inserm, France

Click to download presentation



Coffee break-sponsors




Invited lecturer

Correlating function and gene expression at the level of individual dopamine neurons - form mice to men

Birgit Liss, Ulm University, Germany

 Not available



Microgenomics and Epistemology

Thomas Heams, France




Cell encapsulation for multiplex culture on a chip

Ophelie Berthuy, France

 Not available






Session 2: Microgenomics and DNA

Chairman: Christoph Klein

Chariman: Christoph Klein


Keynote lecturer

Molecular profiling of single circulating tumor cells: Impact for therapy selection and the understanding of cancer evolution

Christoph Klein, Universität Regensburg, Germany

 Not available



Are aptototic circulating tumor cells (CTC) suitable for molecular analysis?

Elisabetta Rossi, Italy




Lunch + posters + sponsors





Circulating tumor cells: from enrichment to single cell sequencing

Pamela Pinzani,University of Florence, Italy

click to download Presentation



Single cell genetic analysis helps validating cytopathological identification of CTC in patients with Clear Cells Renal Carcinoma

Patrizia Paterlini-Brechot,France







Session 3a: Microgenomics and RNA1 : MIQE guideline and microgenomics

Chairman:Mikael Kubista

Chariman: Mikael Kubista


Keynote lecturer

Taking Expression Profiling to New Dimensions

Mikael Kubista,  TATAA Biocenter, Institute of Biotechnology AS CR in Prague, Czech Republic

click to download Presentation



Droplet microfluidics for gene expression study at the single cell level

Davide Ferraro,France

 Click to download presentation



Coffee break-posters




Invited lecturer

Driving Genomics to the Single-Cell Level:  Analysis of RNA Expression Using qPCR and RNAseq

Ken Livak,Fellow Fluidigm Corporation South San Francisco, USA

click to download Presentation


Invited lecturer

Gene expression profiling from Single Laser Capture Microdissected (LCM) Cells 

Stephen Jackson, PhD,Life Sciences Solutions, Thermo Fisher Scientific,South San Francisco, USA




Single-cell transcriptional analysis of FACS-sorted kidney macrophages

Julie Bellière,France

 Not available



Gene expression of autoreactive T cells - Biomarker and pathogenesis of Type 1 diabetes

Anne Eugster,Germany

click to download Presentation



Poster session + French Wine & Cheese





Dinner cruise on the Seine



Friday, May 16 2014






Session 3b: Microgenomics and microRNA and NGS

Chairman:Michael W. Pfaffl

Chariman: Michael W. Pfaffl


Keynote lecturer

Exosome isolation and holistic expression profiling using RNA-Seq and RT-qPCR

Michael W. Pfaffl,Technical University of Münich, Germany


Invited lecturer

Measuring microRNA expression in size-limited samples

Kai Peter Höfig, Munich University, Germany

click to download Presentation



Towards a comprehensive single cell expression profiling

Herbert Auer, Spain

 Not available



A combination of microgenomics approaches to understand the ipmpact of gut microbiota on the regulation of the hypothalamo-pituitary-adrenal axis in rats

Bénédicte Langellier, France




Coffee break - posters




Invited lecturer

Comparative analysis of RNA sequencing methods for degraded or low-input samples

Rahul Satija, Broad Institute of MIT, Massachusetts, USA 

click to download Presentation



Low RNAseq microdissected plant tissue

Bernard Dubreucq, France




GOLD SPONSOR presentation: "Genomic Characterization of Challenging or Limited Research Samples using the Ion AmpliSeq™ Targeted Re-Sequencing Technology"

Dr. Stephen Jackson, Life Technologies/ Life Technologies/Thermo Fischer Scientific, USA












Session 4: Microgrenomics and Proteins

Chairman:Lance Liotta 


Keynote lecturer

Advantages or disadvantages of different methods for proteomics analysis 

Lance Liotta, George Mason University, USA




Oocytomics: combining transcriptomics and proteomics to understand post-transcriptional regulation in bovine oocytes

Rozenn Dalbies-Tran, France

Not available



Combining surface plasmon resonance and mass spectrometry to identify Bone Morphogenetic Protein (BMP) interactants

Catherine Taragnat, France




Coffee break - Posters




Invited lecturer

Mass Spectrometry Imaging coupled to Microproteomics: From  Imaging to identification of proteins on tissue section

Maxence Wisztorski, University of Lille I, France

click to download Presentation


Invited lecturer

Unlocking the prognostic significance of microdissected proximal lesions from human colon

Daniel W. Rosenberg, University of Connecticut Health Center USA

click to download Presentation



Closing remarks