Know more

About cookies

What is a "cookie"?

A "cookie" is a piece of information, usually small and identified by a name, which may be sent to your browser by a website you are visiting. Your web browser will store it for a period of time, and send it back to the web server each time you log on again.

Different types of cookies are placed on the sites:

  • Cookies strictly necessary for the proper functioning of the site
  • Cookies deposited by third party sites to improve the interactivity of the site, to collect statistics

Learn more about cookies and how they work

The different types of cookies used on this site

Cookies strictly necessary for the site to function

These cookies allow the main services of the site to function optimally. You can technically block them using your browser settings but your experience on the site may be degraded.

Furthermore, you have the possibility of opposing the use of audience measurement tracers strictly necessary for the functioning and current administration of the website in the cookie management window accessible via the link located in the footer of the site.

Technical cookies

Name of the cookie


Shelf life

CAS and PHP session cookies

Login credentials, session security



Saving your cookie consent choices

12 months

Audience measurement cookies (AT Internet)

Name of the cookie


Shelf life


Trace the visitor's route in order to establish visit statistics.

13 months


Store the anonymous ID of the visitor who starts the first time he visits the site

13 months


Identify the numbers (unique identifiers of a site) seen by the visitor and store the visitor's identifiers.

13 months

About the AT Internet audience measurement tool :

AT Internet's audience measurement tool Analytics is deployed on this site in order to obtain information on visitors' navigation and to improve its use.

The French data protection authority (CNIL) has granted an exemption to AT Internet's Web Analytics cookie. This tool is thus exempt from the collection of the Internet user's consent with regard to the deposit of analytics cookies. However, you can refuse the deposit of these cookies via the cookie management panel.

Good to know:

  • The data collected are not cross-checked with other processing operations
  • The deposited cookie is only used to produce anonymous statistics
  • The cookie does not allow the user's navigation on other sites to be tracked.

Third party cookies to improve the interactivity of the site

This site relies on certain services provided by third parties which allow :

  • to offer interactive content;
  • improve usability and facilitate the sharing of content on social networks;
  • view videos and animated presentations directly on our website;
  • protect form entries from robots;
  • monitor the performance of the site.

These third parties will collect and use your browsing data for their own purposes.

How to accept or reject cookies

When you start browsing an eZpublish site, the appearance of the "cookies" banner allows you to accept or refuse all the cookies we use. This banner will be displayed as long as you have not made a choice, even if you are browsing on another page of the site.

You can change your choices at any time by clicking on the "Cookie Management" link.

You can manage these cookies in your browser. Here are the procedures to follow: Firefox; Chrome; Explorer; Safari; Opera

For more information about the cookies we use, you can contact INRAE's Data Protection Officer by email at or by post at :


24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal IN-SYLVA France ONF FCBA CIRAD CNPF OFB Université de Rouen

Home page

Tree Cell Engineering Lab (LICA)

Tree Cell Engineering Lab: LICA
Site Orléans: INRAE - Centre Val de Loire - 2163, Avenue de la pomme de pin - CS 4001 Ardon - 45075 Orléans Cedex 2
Vegetative multiplication and plant breeding


Transformation / Genome edition



Phénotypage 1Phénotypage 2



Infrared Microscopy



The LICA is a technical platform inaugurated in 2017 on the Orleans site of the INRAE Val de Loire Center. It is intended to house in a single location and for the duration of studies involving the production and characterization of genetically modified or edited trees. It is a confined building of the L2S2 type that complies with biological safety regulations. Thus, LICA's vocation is to meet the research, training and service needs of public or private research teams involving the production, multiplication and characterization of genetically modified forest resources for experimental purposes and aiming to increase our knowledge of the physiology of forest trees and their capacity to adapt to different environmental constraints. 

LICA brings together the expertise of researchers, engineers and technicians and the equipment of the Integrated Biology Unit for the Development of Tree and Forest Diversity (BioForA) and the Orleans Genetics and Forest Biomass Unit (GBFOR) of INRAE.

Services offer

LICA has a Charter of use and the services offered are presented on its website (

The activities developed at LICA mainly concern :

  • The multiplication and breeding of forest trees from in vitro culture. This activity benefits from laboratories, in vitro culture chambers and automated greenhouses.
  • Genetic transformation by co-culture of Agrobacteria to carry out functional genomic studies mainly in poplar, a model species for forest trees. The proposed techniques allow ectopic expression, expression reduction (by RNA interference) and KO (by genome editing) of the targeted genes.
  • Basic molecular biology techniques to select the most promising events on trees grown under controlled conditions.
  • Phenotyping techniques to evaluate the effects of the modification of the expression of target genes. LICA is particularly well equipped to carry out microphenotyping, especially in wood, the emblematic tissue of trees, thanks to its equipment and skills in histology (fluorescence stereomicroscopy, fluorescence microscopy, laser confocal microcopy, etc.) and molecular imaging (immunolocalizations, in situ hybridization, infrared microspectroscopy).

A support team of four permanent employees, assisted by non-permanent employees, ensures the supervision of the various projects and technological developments carried out in the LICA technical platform. LICA is backed by a BioForA scientific team, an expert in the functional genomics of wood formation. Governance is ensured by the LICA Scientific Council (6 members, including 3 external members).

Operations and data access

LICA's services are open to all public and private research actors. Any questions or requests for collaboration or access to the equipment and services offered by LICA should be addressed to Gilles Pilate. The conditions of access are described in the Charter of Use and on the LICA website. For the design, implementation and management of user projects, LICA undertakes :

  • Provide training and supervision by qualified personnel.
  • Offer efficient and operational installations and equipment.
  • Make available the techniques associated with these materials.
  • Regularly inform users of the progress of their project.
  • To provide the results of experiments as soon as they are available.
  • Respect the confidentiality of the nature and results of the projects carried out.

After examination by the Scientific Committee of the requests submitted concerning their feasibility, we propose the type of project that best meets the requests (collaborative research project, use of equipment by the applicant after training, service provided by the platform's staff). We provide the necessary health and safety supervision for access to and use of LICA laboratories and equipment, in accordance with current French regulations on GMOs.

Data access and cost

Each approved project will be quoted.

Rates differ depending on the type of user:

  • Marginal cost (cost of using labs/equipment and labor excluding public salary) for academic users.
  • Full cost (cost of use of laboratories/equipment and manpower excluding public salary + structural charges) for private users.

The LICA has the following equipment:

  • An in vitro culture laboratory equipped for the preparation and autoclaving of growth media, for transplantation under laminar flow hood and for the rearing of in vitro plants in culture chambers.
  • A molecular biology laboratory equipped for cloning, microbiology (PSM bacteriological hood) and nucleic acid extraction.
  • An experimental greenhouse type S2 (150 m2) with four units for acclimatization and tree breeding. A large unit (75 m2) with a height of 5-6m allows a good development of the aerial part of the trees. A pit of 1 meter depth is also available allowing a good development of the roots.
  • A histology laboratory equipped to carry out sections of plant tissues, their stainings and their observations by various techniques of optical microscopy. For this purpose, the laboratory has a LEICA RM2155 rotary microtome and a Thermo HM 650 vibratome. The laboratory is also equipped with :
    • a Leica fluorescence stereo-microscope which allows the in vivo detection of fluorescent proteins of GFP type
    • an Olympus inverted microscope for observations of cell cultures or tissue sections in transmitted light and epifluorescence
    • a Zeiss confocal microscope for the realization of optical sections and the acquisition of images in multiple fluorescences.
  • An infrared spectroscopy laboratory with hygrometry and temperature control equipped with
    • an infrared spectrometer allowing the acquisition of near (NIR) and medium infrared spectra (MIR, FT-MIR, ATR-FT-MIR).
    • an imager coupled to the spectrometer allowing to realize high resolution infrared images in near and medium infrared