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Reviving freezed-dryed bacteria

The revival of the strains must be done by qualified staff, in an appropriate laboratory with equipment providing aseptic conditions.


  • A diamond tool
  • Pasteur pipettes
  • A Pasteur pipette with round end or a loop


  • 5 ml of peptone nitrated water or other nutrient broth
  • Nutrient agar plates, adapted to the strains

Reviving freezed-dried bacteria:

Strains revival in video:

Make stripes all arround the narrow part of the vial with the diamond tool. 
Sterilize the narrow part of the vial. 
Break the vial in aseptic conditions

Resuspend the lyophilisat with 300µl of the nutrient broth by multiple pipeting. Ensure that the lyophilisat is well resuspended.
Two drops are laid down on the agar plate, the residual bacterial suspension is added to the broth.
One drop is used to spread the inoculum in order to obtain isolated colonies after incubation. 
The other is kept untouched in order to preserve a concentrated inoculum.

Agar plates and tubes are incubated at the optimal temperature for growth.

If no growth occurs on the plates after recommended incubation time, it is possible to spread the nutrient broth on new nutrient agar plates.


Composition of usual media:

peptone nitrated water pH 7.2

Bacto peptone


Distilled water


YPGA medium pH 7,2

Y: Yeast extract (Mérieux)
P: Bacto peptone (Mérieux)


G : Glucose
A : Agar Agar


Distilled water